LOCUS       40924_23709       12189 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB712 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12189)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12189)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12189)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12189)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1178..1201
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     protein_bind    1211..1335
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1372..1402
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1456..2112
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2457..2759
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2803..2927)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2953..3205
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3239..3255)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3263..3279
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3287..3317)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3332..3353)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3389..3641)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3679..4974)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61193.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3679..4974)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5026..5205)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5711..5735)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6256..7044
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7293..7881
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8067..8207)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8551..8745)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8814..9884)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10316..10942)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcgac tacaaggatg acgatgacaa
     1201 ggctgttatc acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     1261 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     1321 tatccagtca ctatggcggc ccctcgaggg gccgcattag gcaccccagg ctttacactt
     1381 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
     1441 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     1501 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     1561 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     1621 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     1681 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     1741 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     1801 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     1861 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     1921 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     1981 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     2041 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     2101 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     2161 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     2221 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     2281 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     2341 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     2401 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     2461 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     2521 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     2581 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     2641 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     2701 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     2761 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     2821 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     2881 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aacctagagc
     2941 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     3001 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     3061 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     3121 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     3181 gcggtgtcat ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatggt
     3241 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3301 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa
     3361 ttaggcgcgc ctatttctat taattcccga tctagtaaca tagatgacac cgcgcgcgat
     3421 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     3481 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     3541 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     3601 ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt cgcctcgagt
     3661 gacttctctg attgcagact atttatacca accagcaaga aagtgtcgaa gtatgaagca
     3721 gaaggcgcag gctagagcct ggaaaacgag aagatggatg caaagagact tttctgattt
     3781 aggaccaaac aacctcaagt aaacattgac gagtgtccca tcttttgtgc cggtcagtaa
     3841 tccggtagca ggatcatacc ttgggagacg atggcgcgga catttcacaa tcccagcaag
     3901 ctgaggaagc gataatagaa ggttcagaac ttgaggaaga aagaagatga ggagggtttc
     3961 actgaagtga cccagaatac caacaactgc catagtcatt ccggcgaaga ctgtgtaagt
     4021 gtctccaaca aaaactgaag aaggatacca attgtaagca agcattgcca aggatgtggc
     4081 catcagcggt tgagtaagaa atatcgagaa ggcatgagct tggtgatact cgggatcaac
     4141 agatgctccg atttgcataa cgttgtgtat caggatagca gcggcaataa caactgtttg
     4201 accgatttca aggccattca gaccagcgtg gatgttaata gagtttgtac aaaagacagc
     4261 aagtagcccc atatataact tatatatcct tcctagatta agcacttcca agccaatata
     4321 agcaacaaga ggttttggta taacaatagt tgtatgacca gcataagcca tcaaaagtgg
     4381 aagggtagca aaagatggca ggacaagttt tactctccaa ggcacatcaa ggacatcatc
     4441 tacaaatcca agcaaaatca tgaagcaaat agatgctagt gctgcattat actccacaag
     4501 ccaattagaa tcttcagtga agttaaaata ctgaaatatt atcgccacga tcaaaaagac
     4561 aataccaaca acaataccca atgactctgg cactttaata tctccttgag gcgtgcctct
     4621 tttgttgata tcaaaaccaa acatgttacg tctcagaacg tatctggcag taacaggaat
     4681 cattttgaga gtgacgaaaa acccaaccaa gctaagcccc gcgttaatta gaatcgatcg
     4741 cttgagttca ttatcaacct tgtagtgaaa acagagaagg tatagataga gcgaacagag
     4801 aagagtagag atcacgaaga tcactcctaa tttcggcggc gcgagacgaa attcctcacc
     4861 ggaagcagaa accgtctttc gggtcatctt ctgctccgat ggcgctgagt tgggttccga
     4921 cgggtccggt ttgttgggaa ttgagatcga tgaagctctc ttacgagctg ccatatctta
     4981 ttgccccccg ggatcagatc tggattgaga gtgaatatga gactctaatt ggataccgag
     5041 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     5101 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     5161 actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     5221 taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc
     5281 tcatgatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     5341 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     5401 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     5461 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     5521 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     5581 ccctgagtgc ttgcggcagc gtgaagctga tccgtcgaga ttaatagaaa taaattcagc
     5641 ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     5701 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     5761 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     5821 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     5881 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     5941 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa
     6001 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     6061 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     6121 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     6181 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     6241 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     6301 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     6361 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     6421 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     6481 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     6541 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     6601 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     6661 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     6721 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     6781 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     6841 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     6901 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     6961 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     7021 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     7081 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     7141 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     7201 ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7261 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7321 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7381 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     7441 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7501 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7561 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7621 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7681 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7741 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7801 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7861 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7921 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7981 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8041 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     8101 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8161 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     8221 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     8281 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     8341 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
     8401 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
     8461 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
     8521 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
     8581 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
     8641 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
     8701 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
     8761 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
     8821 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
     8881 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
     8941 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
     9001 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
     9061 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
     9121 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
     9181 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
     9241 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
     9301 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
     9361 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
     9421 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
     9481 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
     9541 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
     9601 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
     9661 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
     9721 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
     9781 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
     9841 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
     9901 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
     9961 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
    10021 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
    10081 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
    10141 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    10201 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    10261 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    10321 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    10381 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    10441 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    10501 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    10561 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    10621 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    10681 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    10741 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    10801 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    10861 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    10921 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    10981 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    11041 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    11101 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    11161 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    11221 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    11281 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    11341 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    11401 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    11461 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    11521 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    11581 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    11641 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    11701 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    11761 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    11821 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    11881 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    11941 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    12001 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    12061 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    12121 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
    12181 gataaacct
//
